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| Registros recuperados: 13 | |
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| Geffard, A; Geffard, O; Amiard, J; His, Edouard; Amiard Triquet, C. |
| The bioavailability of Cd, Cu, Zn, and Pb from two metal-contaminated sediments (Bidassoa and Dunkerque) was studied using Crassostrea gigas larvae exposed to sediment elutriates. The metal contents within the sediments, the larvae and larval growth, the condition index, and the induction of metallothionein in the larvae were measured. The larval growth and condition index were only affected after exposure to the highest elutriates concentration (5 to 25%) from the most contaminated sediment (Dunkerque). Bioaccumulation of all metals was observed in larvae exposed to Dunkerque elutriatre; only Cu bioaccumulation was observed in the Bidassoa elutriate. The results from larvae exposed to both sediment elutriates show a strong correlation between... |
| Tipo: Text |
Palavras-chave: Metallothionein; Metal bioaccumulation; Larval growth; Crassostrea gigas larvae; Sediment elutriates. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-4748.pdf |
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| MAYA,JUAN DIEGO; RODRÍGUEZ,ANDRÉS; PINO,LAURA; PABÓN,ADRIANA; FERREIRA,JORGE; PAVANI,MARIO; REPETTO,YOLANDA; MORELLO,ANTONIO. |
| Proteins rich in sulfhydryl groups, such as metallothionein, are present in several strains of the parasite Trypanosoma cruzi, the etiological agent of Chagas' disease. Metallothionein-like protein concentrations ranged from 5.1 to 13.2 pmol/mg protein depending on the parasite strain and growth phase. Nifurtimox and benznidazole, used in the treatment of Chagas' disease, decreased metallothionein activity by approximately 70%. T. cruzi metallothionein was induced by ZnCl2. Metallothionein from T. cruzi was partially purified and its monobromobimane derivative showed a molecular weight of approximately 10,000 Da by SDS-PAGE analysis. The concentration of trypanothione, the major glutathione conjugate in T. cruzi, ranged from 3.8 to 10.8 nmol/mg protein,... |
| Tipo: Journal article |
Palavras-chave: Trypanosoma cruzi; Metallothionein; Glutathione; Trypanothione; Benznidazole; Nifurtimox; Buthionine sulfoximine. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100007 |
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| ARMENDÁRIZ,ÁNGELA D; OLIVARES,FELIPE; PULGAR,RODRIGO; LOGUINOV,ALEX; CAMBIAZO,VERÓNICA; VULPE,CHRISTOPHER D; GONZÁLEZ,MAURICIO. |
| The role of metallothioneins (MT) in copper homeostasis is of great interest, as it appears to be partially responsible for the regulation of intracellular copper levels during adaptation to extracellular excess of the metal. To further investigate a possible role of MTs in copper metabolism, a genomics approach was utilized to evaluate the role of MT on gene expression. Microarray analysis was used to examine the effects of copper overload in fibroblast cells from normal and MT I and II double knock-out mice (MT-/-). As a first step, we compared genes that were significantly upregulated in wild-type and MT-/- cells exposed to copper. Even though wild-type and mutant cells are undistinguishable in terms of their morphological features and rates of growth,... |
| Tipo: Journal article |
Palavras-chave: Copper homeostasis; Metallothionein; Microarray. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100015 |
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| Sakulsak,Natthiya. |
| Metallothionein (MT) is a ubiquitous protein with a low molecular weight of 6-7 kDa weight and it was first identified in the kidney cortex of equines as a cadmium (Cd)-binding protein responsible for the natural accumulation of Cd in the tissue. The mammalian MT contains 61 to 68 amino acid residues, in which 18 to 23 cysteine residues are present. The expression of MT starts by binding of metal transcription factor-1 (MTF-1) to the regulative region of MT gene called metal responsive elements (MREs). The induction of MT through the MREs region can be initiated by several metal ions such as zinc (Zn), copper (Cu) and Cd. However, Zn is the only heavy metal which can reversibly and directly activate the DNA-binding activity of MTF-1. In mammals four types... |
| Tipo: Journal article |
Palavras-chave: Metallothionein; Cadmium; Zinc. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000300039 |
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| Vincent-hubert, Francoise; Chatel, Amelie; Gourlay-france, Catherine. |
| We have previously shown that cadmium (Cd) and Benzo[a]pyrene (BaP) induced early DNA damages in zebra mussels, and that the level of DNA strand breaks (SB) returned to a basal level after 3 days of exposure to Cd. The aim of the present study was to go further in the mechanisms of Cd and BaP detoxification. For that purpose, expression of genes encoding for metallothionein (MT), Aryl Hydrocarbon Receptor (AHR), P-gP, catalase, glutathione S-transferase and Heat shock protein 70 (HSP70) proteins have been measured using RT-qPCR. Data reported here show that Cd is a strong inducer of MT and HSP70 genes, and that BaP is a strong inducer of P-gP and AHR genes. Exposure to Cd and BaP resulted in moderate changes in antioxidant enzymes mRNA. Since the increase... |
| Tipo: Text |
Palavras-chave: Benzo[a]pyrene; Cadmium; Metallothionein; Aryl hydrocarbon receptor; Antioxidant enzymes; RT-qPCR. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00184/29547/27873.pdf |
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| Guinand, Bruno; Durieux, Eric D. H.; Dupuy, Celie; Cerqueira, Frederique; Begout, Marie-laure. |
| Growth-related characters, condition factor, and genetic differentiation were investigated for a single cohort of young-of-the-year (YOY) sole within and among nurseries with differing levels of heavy metals (Cd, Cu and Zn) contamination in the two Charentais Straits, Bay of Biscay, France. Analyses were performed when individuals recruited (May), then after a full summer spent in each nursery (October). Levels of phenotypic and genetic diversity were compared, together with genetic differentiation at a candidate metallothionein (MT) locus and three putatively neutral microsatellite loci. No phenotypic or genetic differentiation was detected among nurseries in May, but significant variation at each phenotypic trait and at the multilocus level in October (P... |
| Tipo: Text |
Palavras-chave: Solea solea; Nursery; Bay of Biscay; Metallothionein. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00033/14392/11694.pdf |
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| Geffard, A; Geffard, Olivier; His, Edouard; Amiard, Jc. |
| The bioavailability of Cd, Cu and Zn from a metal-contaminated sediment was studied using Mytilus galloprovincialis embryos and larvae exposed to sediment elutriates, The elutriate concentrations tested were always lower than the threshold at which abnormal larval development appears. The Cd, Cu and Zn contents of the sediment, of the elutriate and of the larvae, as well as the larval growth, the condition index (CI), and the induction of metallothionein (NIT) in the larvae were measured. The Cl was only affected after 96 h of exposure, principally at the highest elutriate concentration (25%, corresponding to 0.1435 mug Cd l(-1) and 14 mug Zn l(-1)). Cd, Cu and Zn bioaccumulation was observed (48 and 96 h), in whole larvae as well as in the cytosolic... |
| Tipo: Text |
Palavras-chave: Sediment elutriate; Mytilus galloprovincialis larvae; Metal bioaccumulation; Metallothionein. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00000/10119/7598.pdf |
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| Gueguen, Yannick; Saulnier, Denis; Santini, Adrien; Magre, Kevin; Garen, Pierre; Bernagout, Solene; Nohl, Marine; Bouisset, Patrick; Helme, Herehia; Planes, Serge; Le Moullac, Gilles. |
| This study was designed to identify in the pearl oyster Pinctada margaritifera, used as a bio-accumulator, molecular biomarkers for the presence of heavy metals in the lagoon environment. Pearl oysters were exposed to 2 concentrations (1 and 10 μg L− 1) of cadmium (Cd) and chromium (Cr) compared to a control. Twelve target genes encoding proteins potentially involved in the response to heavy metal contamination with antioxidant, detoxification or apoptosis activities were selected. P. margaritifera accumulated Cd but not Cr, and mortality was related to the amount of Cd accumulated in tissues. In response to Cd-Cr contamination, metallothionein (MT) was significantly up-regulated by Cd-Cr at both concentrations, while 7 others (SOD, CAT, GPX, GSTO, GSTM,... |
| Tipo: Text |
Palavras-chave: Pinctada margaritifera; French Polynesia; Cadmium; Chromium; Gene expression; Metallothionein. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00376/48707/49146.pdf |
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| Bustamante, Paco; Bertrand, M; Boucaud Camou, E; Miramand, Pierre. |
| The subcellular fractionation of the digestive gland cells of the common cuttlefish Sepia officinalis was performed to investigate the distribution of metals between organelles and cytosol and the different cytosolic fractions separated by gel-filtration chromatography. Total metal concentrations vary over 3 orders of magnitude, ranging from dwt for Pb to dwt for Zn. With the exception of Cd, Co, and Cu, metals were mostly bound to the organelles. Whereas no specific organelle compartment was found for Mn, Ph, and Zn, Fe was mainly associated with nucleus, brown body, and "boule" fraction (i.e., 52%) and 44% of the total Ag was contained in the lysosomal and mitochondria enriched fraction. The link of metals with hydrosoluble proteins in the cytosolic... |
| Tipo: Text |
Palavras-chave: Sepia officinalis; Chromatography; Metallothionein; Detoxification; Trace element; Metal; Cephalopod. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2532.pdf |
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| Registros recuperados: 13 | |
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